GENETIC TOOLS & TECHNIQUES Students investigate the tools and technologies that are used to amplify DNA, identify the genetic profile of organisms and manipulate and modify the genomes of organisms and reasons behind this.
Through teacher facilitated,independent learning, students will undertake a range of activities and utilise several resources in order to further and develop an in depth understanding of knowledge.
INTRODUCTION
Students will be introduced to the concept of using genetic tools and techniques for investigation, e.g. solving a crime.
Lesson One:
Introduce concept of how genetic tools and techniques are used commonly in society; relate to real world examples.
Outline the basic tools of a genetic engineer; restriction enzymes, gel electropohresis, probes, ligase enzymes, vectors and gene cloning.
ACTION
“TOOL”
Cut DNA into fragments at precise locations
Restriction Enzymes
Separate fragments by size
Gel Electrophoresis
Find particular DNA fragments
Probes
Join DNA fragments
Ligase enzyme
Transport DNA into cells
Vectors
Obtain multiple copies of gene
Gene cloning
Explain DNA profiling; use of short tandem repeats and examples of what DNA profiling is used for.
Students are to read article "DNA Technology Today" and investigate a recent case study which utlilises DNA tools. They are to identify what techniques were used and how these contributed to the result of the case study.
GENETIC MANIPULATION: CUTTING DNA INTO FRAGMENTS
Outline to students the fundamental uses of restriction enzymes, gel electrophoresis, probes and ligase enzymes and how these work in tandem to manipulate DNA. Furthermore, extend students understanding through examining gene transformation and PCR.
Lesson Two and Three:
Give overview of how restriction enzymes and gel electrophoresis works and it's purpose (alone and together).
Students are to write the steps of gel electrophoresis including the critical points, and terms (show their understanding).
Complete the activity "Gel Electrophoresis Simulation".
Based on information learnt in lesson two and three, students are to examine the addition of using probes and ligase enzymes to genetic research.
Students are to write a detailed paragraph using examples (through investigating) of the process of manipulating (cutting, seperating, finding and joining back together) DNA.Appropriate terms must be used in the correct manner and all topics addressed.
Lesson Five:
Gene transformation will be examined. Students will "teach themselves this process through the interactive online activity "Harlem DNA Lab: Bacterial Transformations".
GENE CLONING
Students are to gain knowledge of how multiple copies of a gene are made through understanding gene cloning.
Lesson Seven:
Discuss gene cloning, recent advances (medically) and outline the basic process of how it works through diagrams.
Students are to complete activity "Explore more news web-quest". They will investigate genetic cloning and outline the issues related to their chosen topic.
Students investigate the tools and technologies that are used to amplify DNA, identify the genetic profile of organisms and manipulate and modify the genomes of organisms and reasons behind this.
Through teacher facilitated,independent learning, students will undertake a range of activities and utilise several resources in order to further and develop an in depth understanding of knowledge.
INTRODUCTION
Students will be introduced to the concept of using genetic tools and techniques for investigation, e.g. solving a crime.
Lesson One:
- Students are to read article "DNA Technology Today" and investigate a recent case study which utlilises DNA tools. They are to identify what techniques were used and how these contributed to the result of the case study.
DNA Technology Today articleGENETIC MANIPULATION: CUTTING DNA INTO FRAGMENTS
Outline to students the fundamental uses of restriction enzymes, gel electrophoresis, probes and ligase enzymes and how these work in tandem to manipulate DNA. Furthermore, extend students understanding through examining gene transformation and PCR.
Lesson Two and Three:
- Give overview of how restriction enzymes and gel electrophoresis works and it's purpose (alone and together).
- Students are to write the steps of gel electrophoresis including the critical points, and terms (show their understanding).
- Complete the activity "Gel Electrophoresis Simulation".
Gel Electrophoresis Simulation Activity and **handouts**Lesson Four:
Lesson Five:
- Gene transformation will be examined. Students will "teach themselves this process through the interactive online activity "Harlem DNA Lab: Bacterial Transformations".
Harlem DNA Lab: Bacterial Transformations web pageLesson Six:
- General discussion of PCR and the key steps involved. Provide diagram of the process.
Diagram of PCR process- To extend students understanding, they will undertake a practical activity and complete a PCR.
Practical PCR activityGENE CLONING
Students are to gain knowledge of how multiple copies of a gene are made through understanding gene cloning.
Lesson Seven:
- Discuss gene cloning, recent advances (medically) and outline the basic process of how it works through diagrams.
- Students are to complete activity "Explore more news web-quest". They will investigate genetic cloning and outline the issues related to their chosen topic.
Explore More News Web Quest Activity